Chromatography

Chromatography:
Using chemicals to extract the pigment.

Aim: To extract the pigment.

Hypothesis:
I predict that the mixture will
Equipment:

Method:

Get leaves from three different trees.

Leaves.

1st Leaf

2nd Leaf

3rd Leaf

Tear it up into tiny pieces.

.

Put all the pieces straight into the mortar and pestle.

Start grinding.

Once it has reached a pasty texture add Ethanol using a dropper.

Transfer the mixture to a test tube and add more Ethanol if needed.

Repeat these step with the other two leaves.
Place all the test tubes into the test tube rack.

Wait overnight and see what happens.

Cut your filter paper into long strips and mark out a line on both sides.

Break your splint in half

Tape your splint onto the filter paper.

.

Add a drop of you leaf mixture onto the line that you drew.

Add Ethanol to one of your boiling tubes low enough so it doesn't go past the line.

Insert the paper with the droplet onto the tube.

Wait for the result.




Results:
There wasn't a lot of movement in terms of our mixture, however, Mr Guy's mixture was very visible.
We decided to do it again due to our pigment not really showing, next time we should probably have less ethanol and more crushed leaves.

Mr Guy's Mixture

We decided to do it again but instead of using leaves as solute we used felt pens we also used water as the solvent instead of ethanol.
I knew to use dark colours because I know it has the most pigments and has a lot of colour mix into it.

Aim: To compare this experiment to the other one.

Equipment

Results:
The results were much more visible than the leaves, you can see the colour move up clearly and it was very vivid.

The solvent (water) moves up the chromatography paper and as it does it takes some of the pigment of the solute (felt pen) and spread it on the paper. The colours are separated due to it being different weight and the lighter colours get further up the paper.

I think this experiment worked well better than the leaves because the felt pen was more concentrated.